show Abstracthide AbstractWe proposed to uncover the RNome of a saprophytic strain ofL. biflexa, by a systematic RNomic approach. Total transcriptome of non-pathogenic L. biflexa will be identified through differential(dRNA-seq) transcriptomic mapping. TSS profile and differential RNA expression will be annotated. Importantly, we will compare thetranscriptome profile of L. biflexa with those of L. interrogans to identify the RNA candidates (mRNA and regulatory sRNA) that areunique to pathogenic and saprophytic leptospira.